The Anti-CD3 antibody is designed to react with intracytoplasmic portions of the CD3 antigen through T-cells. It works to stain the human T-cells in the medulla and cortex of the peripheral lymphoid tissues and thymus. It can be used for neoplastic and normal T-cells.
More Information
The Anti-CD3 antibody is designed to be used for research. There are three types of this antibody, including the polyclonal version with no clone, and two monoclonal versions with a clone of SP162 and SP7.
The immunogen for the polyclonal and monoclonal versions are the synthetic peptide (13-mer) that corresponds to the internal region of an epsilon chain of the protein. The isotype is the Rabbit IgG, and it has an undetermined epitope. Likewise, all versions have a molecular weight of 19kDa.
Applications
The polyclonal version uses Immunohistochemistry and Western Blotting while the monoclonal versions can use those two options or Flow Cytometry.
For the IHC procedure, you can use paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides are required. You can find a concentrated and pre-diluted formula for all versions. If you are using a concentrate, you should dilute the antibody using a ratio of 1:200 for polyclonal, 1:150 for the monoclonal versions.
For all Anti-CD3 options, you will retrieve the antigen by boiling the tissue section in a 10mM citrate buffer with a pH of 6.0. Do this for 10 minutes and then let it cool to room temperature for 20 minutes. Likewise, all versions should have an incubation period of 10 minutes at room temperature. The positive control for IHC testing is the tonsil.
For Western Blotting, you should dilute the concentrated antibody using a ratio of 1:25 and incubate for one hour at room temperature. The positive control is the Jurkat cell lysate.
The Anti-CD3 antibody is helpful to stain T-cells in human tissue for testing. Visit Spring Bioscience at to learn more.
